GE Oven RPN2510E User Manual

GE Healthcare  
Amersham  
Hybridization oven/shaker  
Product User Manual  
Codes:  
RPN2510E  
RPN2511E  
RPN2512E  
 
1. Legal  
GE, imagination at work and GE monogram are trademarks of General Electric  
Company.  
Amersham, Alkphos Direct, ECF, ECL, ECL Plus, ECL Advance, Hybond,  
Hypercassette, Hyperfilm, Hyperscreen, Hypertorch, Megaprime, Nick, Rapid-  
hyb, Ready-To-Go, Rediprime, Sensitive and Trackertape are trademarks of GE  
Healthcare companies.  
All third party trademarks are the property of their respective owners.  
© 2003–2007 General Electric Company – All rights reserved.  
First published November 2003  
All goods and services are sold subject to the terms and conditions of sale of the  
company within GE Healthcare which supplies them.  
A copy of these terms and conditions is available on request. Contact your local  
GE Healthcare representative for the most current information.  
GE Healthcare UK Limited.  
Amersham Place, Little Chalfont,  
Buckinghamshire, HP7 9NA UK  
RPN2510EUM Chapter 1 Rev C 06/2007  
3
 
2. Introduction  
One of the most widely used techniques in the molecular biology field is the  
immobilization of DNA and RNA onto a solid support membrane and subsequent  
hybridizations with a specific single stranded probe, labeled to facilitate its  
detection.  
Using the GE Healthcare Hybridization Oven/Shaker ensures that the temperature  
and shaking/rotation frequency, and hence the stringency of hybridization and  
washing steps are rigidly controlled. This enables rapid and reproducible probing of  
nucleic acids, and proteins immobilized on nylon and nitrocellulose membranes.  
The GE Healthcare Hybridization Oven/Shaker is a multipurpose instrument  
combining accurate temperature control with a choice of interchangeable  
hybridization modes:  
• Variable speed rotisserie: holding 7 × 40 mm or 2 × 75 and 2 × 40 mm  
hybridization bottles.  
• A variable speed platform shaker for ‘box’ hybridizations, depurination,  
denaturation and neutralization steps.  
The instrument is:  
Economical: bottle hybridization minimizes probe volumes, reducing reagent  
volumes and enhancing signal intensity.  
Precise: stringency of hybridization and washing steps are rigidly controlled,  
ensuring reproducible results.  
Sensitive: validated protocols ensure optimal hybridization and washing steps,  
enhancing multiple reprobing when using Hybond™ membranes.  
Safe: the double-glazed polycarbonate/acrylic door offers excellent thermal  
insulation whilst minimizing radiation exposure.  
Convenient: small foot-print maximizes the use of limited laboratory space.  
The instrument is suitable for use in conjunction with radiolabeled probes  
using Rapid-hyb™ buffer, non-radioactive nucleic acid labeling and detection  
systems such as AlkPhos Direct™, and protein labeling and detection systems  
including ECL™, ECL Plus™ and ECL Advance™. Some protocols for use with these  
applications are included in section 7.  
RPN2510EUM Chapter 2 Rev C 06/2007  
4
 
 
3. Safety warnings and  
precautions  
If the equipment is not used in the manner described in this manual the  
protection provided might be impaired  
This equipment is designed to operate under the following conditions: -  
For indoor use only  
Use in a well ventilated area  
Ambient temperature range + 5°C to + 40°C  
Altitude to 2000 m  
Relative humidity not exceeding 80%  
Mains supply fluctuation not exceeding 10%  
Over-voltage category II IEC60364-4-443  
Pollution degree 2  
Use with a minimum distance all around of 200 mm from walls or other items  
The unit should be carried using both hands.  
Never move or carry the unit when in use or connected to the mains electricity supply.  
In the case of mains interruption, a fault or electrical failure, the unit will continue  
to operate on restoration of the electricity supply or removal of the fault.  
3.1. Electrical Installation  
WARNING: Lethal voltages inside the Hybridization Oven casing. Always  
switch the Hybridization Oven/Shaker off and remove the plug from the  
electrical socket before performing any cleaning or maintenance of the  
instrument.  
THIS INSTRUMENT MUST BE  
EARTHED  
IMPORTANT: The Hybridization Oven/Shaker has been supplied with a cordset and  
plug. Check that the appropriate plug for your electrical supply has been included.  
If there is any doubt consult a qualified electrician and inform your local GE  
Healthcare sales office.  
Should the plug require replacement in the future, the following information should  
be noted:  
The wires in the mains lead are coloured in accordance with the following code:  
GREEN/YELLOW  
BLUE  
BROWN  
Earth  
Neutral  
Live  
As the colours of the wires in the mains lead of this item may not correspond with  
the coloured markings identifying the terminals in your plug, proceed as follows:  
• The wire which is coloured green/yellow must be connected to the terminal in  
the plug which is marked with the letter ‘E’ or by the earth symbol or colours  
green or green/yellow.  
• The wire, which is coloured blue, must be connected to the terminal, which is  
marked with the letter ‘N’ or coloured black.  
• The wire, which is coloured brown, must be connected to the terminal, which is  
marked ‘L’ or coloured red.  
• Fit the plug with the appropriate fuse. Always replace the plug cover, never use  
the plug without the cover.  
The GE Healthcare Hybridization Oven/Shaker is intended for research use only  
and not for any other purpose.  
IF IN DOUBT CONSULT A QUALIFIED  
ELECTRICIAN  
RPN2510EUM Chapter 3 Rev C 06/2007  
5
 
 
4. Specification  
Overall dimensions  
Height:  
Depth:  
Width:  
17”  
15”  
15”  
(43.5 cm)  
(38.0 cm)  
(38.0 cm)  
Oven dimensions  
Height:  
Depth:  
Width:  
8”  
9”  
10”  
(20.0 cm)  
(23.0 cm)  
(25.0 cm)  
Weight:  
24 kg  
18 litres  
Capacity (nominal):  
Temperature range:  
Temperature precision:  
Temperature fluctuation:  
Power rating:  
Ambient plus 5°C–80°C  
+/- 0.5°C  
+/- 0.1°C (@37°C)  
250 W  
Over temperature cut-out:  
Temperature variation:  
Rotisserie speed:  
1°C over set temperature  
< 0.25°C  
2–10 rev/minute  
5–70 strokes/minute  
Shaker platform speed:  
Electrical  
Nominal Voltage/Hertz/Amp  
Product code  
230 V / 50 Hz / 3.1 A  
110/120 V / 60 Hz / 4 A  
100 V / 50/60 Hz / 4 A  
RPN2510E  
RPN2511E  
RPN2512E  
Each instrument is supplied with 1 rotisserie (RPN2514),  
6 hybridization bottles (RPN2516) and an instruction manual.  
Accessories  
Rotisserie, holds 7 × 40 mm hybridization bottles  
RPN2514  
RPN2515  
RPN2516  
RPN2517  
RPN2518  
RPN2519  
Rotisserie, holds 2 × 75 mm and 2 × 40 mm hybridization bottles  
Hybridization bottle, 260 × 40 mm (For rotisserie RPN2514, pack of 6)  
Hybridization bottle, 170 × 40 mm (For rotisserie RPN2514, pack of 6)  
Hybridization bottle, 240 × 75 mm (For rotisserie RPN2515, pack of 2)  
Hybridization mesh, 1 roll 21.5 cm × 10 m  
RPN2510EUM Chapter 4 Rev C 06/2007  
6
 
 
5. Setting up the Hybridization  
Oven/Shaker  
1. Remove all packaging and place the Hybridization Oven/Shaker on a level  
working surface, ensuring that there is sufficient room above the instrument to  
allow the door to be opened fully.  
2. Leave the unit to stand for a minimum of 3 hours. This will allow acclimation to  
the new ambient temperature.  
3. Plug the female end of the power cable into the Hybridization Oven/Shaker.  
4. Connect the power cable to a suitably grounded electrical outlet. The correct  
operating voltage of the Hybridization Oven/Shaker is found on the product  
information label on the rear of the instrument.  
5. Turn the Mains ON/OFF switch (1 on Fig 1) to the ON position.  
6. The GE Healthcare Hybridization Oven/Shaker is now ready for use.  
1
2
3
4
5
6
7
Rotisserie  
45  
30  
Temperature (°C)  
6
4
60  
8
on  
off  
press  
to set  
on  
off  
15  
2
0
10  
5
70  
rotisserie  
(rev/minute)  
shake r  
(strokes/minute)  
Hybridisation Oven/Shaker  
Fig 1. Diagram of instrument control  
panel  
5.1. Setting the oven temperature  
1. The Oven temperature controls are located on the right hand side of the control  
panel (numbers 4 to 7 in figure 1).  
Rotisserie/shaker controls  
1. Rotisserie/shaker ON/OFF button  
2. Rotisserie/shaker speed selector dial  
3. Motor on indicator  
2. To turn the temperature control ON press the on / off button (4). The LED display  
(6) will show the current Oven temperature.  
Temperature controls  
4. Temperature on/off button  
5. Press to set button  
6. LED temperature display  
7. Temperature selector dial  
3. To set the temperature, press and hold the press to set button (5) and at  
the same time rotate the temperature selector dial (7) until the required  
temperature is shown on the digital display (6).  
4. Release the press to set button (5) and the temperature display (6) will revert  
back to the actual Oven temperature.  
5. The set temperature can be viewed at any time simply by pressing and holding  
the press to set button (6)  
6. The oven will now automatically heat up to the set temperature.  
Note: the Oven is fitted with automatic  
digital over-temperature protection (see  
Section 8.1. for more details).  
5.2. Setting up the rotisserie  
The rotisserie is installed in the Hybridization Oven/Shaker during transit. To use  
the rotisserie for bottle hybridization, the following procedure should be adopted:  
1. Lift up the oven door to its fullest extent to allow complete access to the oven  
interior.  
2. Lift the rotisserie vertically out of the oven. Unpack and place on the bench.  
NOTE: Always ensure that the weight  
is evenly distributed on both sides  
of the rotisserie. Place an empty  
hybridization bottle into the other side  
of the rotisserie as a counterbalance if  
necessary.  
3. Place the membranes to be hybridized into the required number of hybridization  
bottles. Using the rotisserie as a stand for the bottles, place the bottles into the  
rotisserie, pushing them down as far as they will go.  
RPN2510EUM Chapter 5 Rev C 06/2007  
7
 
 
4. Place the rotisserie into the oven onto the rotation mechanism, ensuring that  
the serrated bands at either end of the rotisserie locate onto the steel cogs  
of the rotation mechanism at the rear of the oven. (B on figure 2). The plastic  
flanges of the rotisserie locate on to the small wheels on the oven floor. Close  
the oven door.  
5. Ensure that the desired temperature has been set (see section 5.1).  
6. The speed controls are on the left hand side of the control panel (numbers 1 to  
3 in figure 1). Turn the Rotisserie/Shaker ON by pressing the on / off button (1).  
The red indicator light (3) above the speed selector dial (2) will illuminate.  
7. Turn the Rotisserie/Shaker speed selector dial (2) clockwise until the desired  
rotation speed is reached (allowable values are 2–10 rpm).  
The rotisserie will now start to rotate at the set speed.  
8. When hybridization is complete turn the Rotisserie/Shaker OFF by pressing the  
on / off button (1). The red indicator (3) will go out.  
5.3. Setting up the platform shaker  
During transit, the platform is stored vertically at the rear of the oven chamber. It  
can remain in this position whilst the rotisserie is in use. To use the platform shaker  
for ‘sandwich box’ hybridizations, the following procedure should be adopted:  
1. Open the oven door to its fullest extent, lift the rotisserie vertically and store in a  
safe place.  
2. Lift the platform by its handle (A, see figure 2) from its storage position, slide it  
forward and locate it on the rocking mechanism by placing the side pegs of the  
platform into the retainers (D) on the side walls of the oven chamber. This action  
seats the nylon blocks (C) on the underside of the platform on to the pegs (E)  
protruding from the rocker mechanism at the rear of the oven.  
3. Place the box in which the hybridization is being performed on to the shaker  
platform and close the oven door.  
4. Ensure that the desired temperature has been set (see section 4.1).  
5. Turn the Rotisserie/Shaker ON by pressing the on / off button (1). The red  
indicator light (3) above the speed selector dial (2) will illuminate.  
6. Rotate the Rotisserie/Shaker speed selector dial (2) clockwise, until the desired  
shaker speed is reached. Allowable values are 5–70 strokes per minute.  
The shaker platform will now oscillate at the set speed.  
7. When hybridization is complete turn the Rotisserie/Shaker OFF by pressing the  
on / off button (1). The red indicator will go out (3).  
B
A
B
C
C
Fig 2. Hybridization oven drive  
components  
D
D
E
RPN2510EUM Chapter 5 Rev C 06/2007  
8
 
 
6. Hybridization using the platform  
shaker  
The Hybridization Oven/Shaker is compatible with the hybridization technologies  
available from GE Healthcare. These include radioactive hybridizations using  
Rapid hyb buffer and the range of non-radioactive systems for the labelling and  
detection of proteins and nucleic acids (see appendix 1).  
When using the platform shaker the hybridization and washing conditions  
recommended in the appropriate GE Healthcare literature should  
be used. The following protocol therefore provides a guideline. For specific  
hybridization times and temperatures, refer to the relevant protocol booklet.  
1. Prepare blots as recommended in the appropriate Hybond protocol booklet.  
2. Set the oven temperature as described in section 5.1.  
3. Place the membrane in a suitable box (or bag) and cover with sufficient  
prehybridization buffer to ensure that the entire surface of the membrane is  
covered. Recommended volume: surface area ratio is given in most protocol  
booklets. Seal the box (or bag) securely.  
4. Place the box (or bag) on the platform, set the oscillation speed to 30 strokes/  
minute as described in section 5.3. Prehybridize for the required length of time.  
5. Remove the box (or bag) from the oven and carefully add the labeled single  
stranded probe (denaturation may be required post labelling refer to the  
appropriate protocol booklet) to the prehybridization buffer.  
NOTE: Do not pipette the probe solution  
directly on to the membrane as this  
may cause localized background.  
6. Reseal the box (or bag) securely, replace it on the platform and hybridize for the  
required length of time.  
7. Remove the membranes and place in a clean box containing the first stringency  
wash solution.  
8. Increase the oscillation speed of the platform to 60 strokes/minute. Carry out  
the recommended washing protocol at the appropriate temperature and for the  
appropriate times.  
RPN2510EUM Chapter 6 Rev C 06/2007  
9
 
 
7. Hybridization using the rotisserie  
Several advantages are associated with performing hybridizations in bottles,  
namely those of safety and economy as outlined in the introduction. However,  
the use of bottles for hybridizations and washing procedures requires certain  
adaptations to standard protocols.  
7.1. Assembly of membranes into bottles  
1. Add approximately 20 ml 2 × SSC buffer into the hybridization bottle. The  
rotisserie acts as a convenient bottle stand.  
2. Pre-wet the membrane in 2 × SSC buffer and loosely roll it up.  
3. Insert the rolled up membrane into the bottle and replace the cap. Ensure  
that the cap is screwed on securely, (hand tight plus a quarter turn). DO NOT  
OVERTIGHTEN, or the thread of the cap can be damaged, leading to leakages.  
NOTE: If placing several small blots into  
one bottle, prewet the membranes as  
above, and space them out along the  
length of the bottle with forceps.  
4. Place the bottle on a flat surface and roll it gently in the opposite direction to  
that in which the membrane is coiled. This rolling action causes the membrane  
to uncoil, so lining the inner surface of the bottle.  
NOTE: The use of a mesh in bottle  
hybridizations to ensure uniform  
contact between the membrane  
surface and the buffer has been  
recommended.  
However, studies at GE Healthcare laboratories using a wide range of hybridization  
mesh technologies demonstrate a resulting loss of sensitivity due to partial  
absorption of the probe into the mesh.  
A nylon mesh (RPN2519) is available as an optional extra, as it can facilitate easier  
handling of a number of blots and the more fragile nitrocellulose membranes.  
These handling advantages should be considered against the potential loss of  
sensitivity before use.  
When a hybridization mesh is used in conjunction with the membrane, the  
following procedure should be adopted:  
5. Pre-wet the mesh alongside the membrane in 2 × SSC buffer and place the  
prewetted membrane on top of the mesh. The mesh should be slightly larger  
than the blot in all dimensions. Roll both up together, with the mesh on the  
outside of the membrane, and insert into the bottle as described above (7.1.4.).  
7.2. Hybridization  
1. Set the required oven temperature as detailed in section 5.1.  
2. Discard the 2 × SSC, used in the bottle to unroll the membrane, and replace  
with prehybridization buffer. Recommended volumes are provided in most GE  
Healthcare protocol booklets. Generally a minimum of 10–15 ml per 20 × 20 cm  
blot is advised. Seal the bottle, avoiding overtightening.  
3. Place the hybridization bottle(s) into the rotisserie (as detailed in section 5.2.) add  
counterbalance bottles if necessary. Place the rotisserie into the oven so that  
the bottles are rotating in the same direction as the membrane is rolled, see  
Figure 3.  
Fig 3. Rotation of rotisserie  
rotation of  
membrane  
RPN2510EUM Chapter 7 Rev C 06/2007  
10  
 
 
4. Prehybridize the membrane for the specified length of time at a rotisserie speed  
of 4 rpm.  
5. Following prehybridization, turn off the rotisserie, remove the rotisserie from the  
oven. Add the labeled probe to the buffer, either by removing an aliquot of the  
buffer, adding the probe and returning the aliquot to the bottle; or by adding the  
probe directly into the bottle, avoiding the membrane.  
6. Hybridize for the specified length of time at a rotisserie speed of 4 rpm.  
7.3. Membrane washing procedures  
Membranes can either be removed from the hybridization bottles and washed in a  
box on the platform shaker (this is a more efficient procedure), or the washing  
procedure may be carried out in the bottles. If the platform shaker is used, follow  
the standard washing procedure mentioned in the appropriate protocol booklet.  
If bottles are to be used it is necessary to modify the standard washing procedure.  
Outlined in this section are the optimized washing protocols for radioactive  
hybridizations and non-radioactive based hybridizations.  
7.3.1. Radioactive Hybridizations  
1. Carefully drain off the hybridization buffer, rinse the bottle and the membrane  
thoroughly with 2 × SSC and discard.  
NOTE: This last step is a high stringency  
wash and should be omitted if related  
sequences are to be probed.  
2. Perform the following stringency washes in large volumes (100 ml minimum) of  
the following solutions, at a rotisserie speed of 8 rpm:  
2 × 10 minutes with 2 × SSC, 0.1% SDS at 65°C  
1 × 15 minutes with 1 × SSC, 0.1% SDS at 65°C  
2 × 10 minutes with 0.1 × SSC, 0.1% SDS at 65°C  
(More washes over the same time period for each stringency condition can  
improve background).  
3. Remove the membrane from the bottle, drain off excess stringency wash, wrap  
in SaranWrap™ and autoradiograph.  
7.3.2. AlkPhos Direct Hybridizations  
4. Drain off the hybridization buffer, rinse the bottle and the membrane thoroughly  
with primary wash buffer and discard.  
5. Perform the following stringency washes in large volumes (100 ml minimum) of  
the following solutions, at a rotisserie speed of 8 rpm:  
3 × 10 minutes with primary wash buffer solution at 55°C  
NOTE: The room temperature washes  
can be achieved by switching off  
the oven and leaving the door open  
whilst performing the washes or by  
allowing the oven to cool down to room  
temperature before performing the  
final washes.  
3 × 5 minutes with secondary wash buffer at room temperature  
6. Remove the membrane from the bottle and detect using the standard  
procedures outlined in the protocol booklet.  
RPN2510EUM Chapter 7 Rev C 06/2007  
11  
 
 
8. Maintenance/care/cleaning of  
the oven/shaker  
The GE Healthcare Hybridization Oven/Shaker is designed to provide trouble-free  
operation. The base of the oven and the shaker tray act as a spills tray and will  
contain any spillage that occurs during hybridization and washing procedures.  
To ensure lasting operation the following instructions should be followed:  
ALWAYS DISCONNECT THE HYBRIDIZATION OVEN/SHAKER FROM THE  
ELECTRICAL SUPPLY BEFORE CLEANING OR DRYING THE INSTRUMENT.  
1. Any leakage from the hybridization bottles or the sandwich boxes should be  
cleaned up immediately. Do not allow any liquids to enter the drive mechanism.  
2. Wipe away any liquids from inside and outside of the unit using soap and water  
with a soft cloth or sponge.  
3. Do not allow chemicals to remain on unit surfaces.  
4. Never clean unit with abrasive pads or cleaners.  
5. Never clean unit with acetone or chloroform.  
Only spare parts supplied or specified by GE Healthcare or its agent should  
be used. Fitting of non-approved parts may affect the performance of the  
safety features designed into the instrument.  
8.1. Error codes/faults  
The Oven has built in fault diagnostics. If a fault occurs, this system will display  
an error code in the LED display to help the service engineer rectify the problem.  
Please see the table below for details of error codes and other faults. In the  
unlikely event that a problem does occur, note down which code / fault you  
observe and contact your nearest GE Healthcare Office for further assistance. If  
you have your own service personnel available for repair, a comprehensive service  
manual is available on request.  
Fault  
Safety system in operation  
Fault displayed by  
Temperature probe PT100 reading low  
Automatic over-temp system activated  
LED displays Err and 0.1 alternatively  
LED displays Err and 0.2 alternatively  
Top left dot flashes in display  
Temperature probe PT100 reading high Automatic over-temp system activated  
Triac fault  
Secondary relay control activated  
Cuts power to motor  
Rotisserie / shaker motor stalled  
No movement seen but Rotisserie red  
indicator light still on  
Software crash  
Cuts mains power  
No temperature or motor motor power,  
all LED’s blank  
RPN2510EUM Chapter 8 Rev C 06/2007  
12  
 
 
9. Troubleshooting guide  
This section briefly summarizes some of the potential problems encountered during membrane hybridizations. More  
complete troubleshooting guides are supplied in the pack leaflet that accompany each product.  
Symptom  
Cause  
Remedy  
1. Membrane curling up in  
hybridization bottle  
1.1. Incorrect orientation of bottle in  
1.1. Ensure membrane is rolled up in  
the same direction as the bottle is  
rotating (see section 7.2)  
rotisserie  
2. High background  
2.1. Probe concentration too high  
2.1. Reduce probe concentration  
32  
2.2. Unincorporated P nucleotides not 2.2. Remove unincorporated  
removed  
nucleotides  
2.3. Insufficient blocking  
2.3. Use recommended hybridization  
buffer or extend prehybridization  
time  
2.4. Insufficient washing  
2.4. Increase number of buffer changes  
during the washing stage or  
increase stringency of final wash  
3. Weak signal  
3.1. No transfer from gel to membrane  
3.1. Load extra lanes with control DNA.  
Transfer can be checked by  
restaining gel with ethidium  
bromide. If large DNA fragments  
are detected poorly, use a  
depurination step (0.25 M HCI)  
3.2. Probe not labeled  
3.2. Check probe labelling before  
hybridization. See protocol in probe  
labelling booklet  
3.3. Probe not denatured  
3.3. Boil probe for 5 minutes before  
adding to hybridization buffer  
3.4. Low specific activity of probe  
3.5. Washes too stringent  
3.4. Review labelling conditions  
3.5. Increase buffer salt concentration  
and decrease temperature  
4. Patchy backgrounds  
4.1. Hybridization buffer or wash  
solution not evenly covering  
membrane  
4.1. Increase volume of hybridization  
wash or solutions or use mesh (see  
section 7.1. step 4)  
4.2. Damaged membrane  
4.2. Handle membrane carefully with  
forceps  
5. High background around edge of  
membrane  
5.1. Damaged membrane  
5.1. Use clean scissors or a sharp  
scalpel to cut membrane  
RPN2510EUM Chapter 9 Rev C 06/2007  
13  
 
 
Appendix 1. Products for  
electrophoresis  
DNA Markers  
Precise Sizing  
Digest of Natural DNAs  
50 Base-pair 100 Base-pair  
KiloBase  
DNA marker  
27-4004-01  
DRigest III  
27-4060-01  
ladder  
ladder  
27-4005-01  
27-4007-01  
500a  
450  
400  
350  
300  
250  
200  
150  
100  
50  
2000a  
1900  
1800  
1700  
1600  
1500  
1400  
1300  
1200  
1100  
1000  
900  
10000  
8000  
6000  
5000  
4000  
3000  
2500  
2000  
1500  
1000  
500  
23130b  
9416  
6557  
4361b  
2322  
2027  
1353  
1078  
872  
603  
564d  
310  
800  
281  
700  
271  
600  
234  
500  
194  
400  
125c  
118  
300  
200  
72  
100  
a not necessarily the largest size fragment possible, only the largest that is  
readily distinguishable  
b cos ends are located on these bands  
c may not always be visible  
Marker code number  
27-4005-01  
Recommended gel  
2% agarose/6% polyacrylamide  
1.5% agarose  
Loading amount (µg/lane)  
Heat before loading  
2.0  
2.0  
No  
27-4007-01  
No  
No  
27-4004-01  
0.8% agarose  
0.5  
27-4060-01  
1% agarose  
0.5–1.0  
60–65°C for 2 minutes  
RPN2510EUM Appendix 1 Rev C 06/2007  
14  
 
 
Appendix 2. Hybond membranes  
for nucleic acid applications  
Hybond ECL Hybond C Extra Hybond P  
Size  
Pack size  
Hybond N+  
Hybond XL  
Hybond N  
Hybond NX  
82 mm  
50 discs  
50 discs  
RPN82B  
RPN87B  
RPN82S  
RPN87S  
RPN132S  
RPN137S  
RPN82N  
RPN87N  
RPN132N  
RPN137N  
RPN82T  
RPN87T  
RPN82D  
RPN82E  
87 mm  
132 mm  
50 discs  
RPN132B  
RPN137B  
RPN1782B  
RPN1787B  
RPN1732B  
RPN1737B  
RPN3050B  
RPN132T  
RPN137T  
RPN132D  
137 mm  
50 discs  
RPN137E  
82 mm  
50 gridded discs  
50 gridded discs  
50 gridded discs  
50 gridded discs  
5 sheets  
87 mm  
132 mm  
137 mm  
30 × 50 cm  
9 × 10 cm  
10 × 10 cm  
15 × 20 cm  
20 × 20 cm  
22.2 × 22.2 cm  
14 × 16 cm  
12 × 10 cm  
15 × 10 cm  
6 × 8 cm  
RPN3050S  
RPN3050N  
10 sheets  
10 sheets  
10 sheets  
10 sheets  
10 sheets  
15 sheets  
20 sheets  
20 sheets  
50 sheets  
50 sheets  
50 sheets  
50 sheets  
50 sheets  
50 sheets  
1 roll  
RPN910D  
RPN1010D  
RPN1520D  
RPN2020D  
RPN1520B  
RPN2020B  
RPN2222B  
RPN1520S  
RPN2020S  
RPN2222S  
RPN1520N  
RPN2020N  
RPN2222N  
RPN1520T  
RPN2002T  
RPN2020E  
RPN2020F  
RPN1416F  
RPN1210B  
RPN1510B  
RPN1210S  
RPN1510S  
RPN1210N  
RPN1510N  
RPN68D  
RPN78D  
7 × 8 cm  
11.9 × 7.8 cm  
22.2 × 22.2 cm  
22.5 × 22.5 cm  
115 × 73 mm  
20 cm × 3 m  
30 cm × 3 m  
30 cm × 3 m  
RPN119B  
RPN2250B  
RPN225B  
RPN1576B  
RPN203B  
RPN303B  
RPN119S  
RPN119N  
RPN203S  
RPN303S  
RPN203N  
RPN303N  
RPN203D  
RPN303D  
RPN3032D  
RPN203E  
RPN303E  
1 roll  
RPN303T  
RPN303F  
a
1 roll  
a = 0.2 µm pack size  
RPN2510EUM Appendix 2 Rev C 06/2007  
15  
 
 
Appendix 3. Radioactive labeling  
systems  
Labelling  
system  
Technology  
Nucleotide  
Amount of  
template  
Labelling  
time  
Probe specific  
activity  
Recommended  
application  
Product  
code  
(dpm/µg)  
Rediprime  
II  
Random-prime  
Random-prime  
dCTP only  
dCTP only  
25 ng  
10 minutes  
5 minutes  
2
2
×
×
109  
109  
membrane  
hybridization  
RPN1633  
RPN1634  
Ready-To-Go  
DNA labelling  
beads  
10 ng–1 µg  
membrane  
hybridization  
DNA labelling  
27-9240-01  
Megaprime  
Random-prime  
Nick translation  
any dNTP  
any dNTP  
25 ng  
1 µg  
10 minutes  
2–3 hours  
2
2
×
×
109  
109  
membrane  
hybridization  
RPN1604  
RPN1605  
RPN1606  
RPN1607  
Nick translation  
production of  
large amounts  
of probe  
N5000  
N5500  
RPN2510EUM Appendix 3 Rev C 06/2007  
16  
 
 
Appendix 4. Non-radioactive  
labeling and detection systems  
Labelling and detection  
system  
Sensitivity  
Time from  
hybridization light output  
to detection  
Duration of  
Strip before Quantification Recommended application  
re-probing  
AlkPhos Direct (RPN3690)  
ECL Direct (RPN3000)  
0.06 pg  
0.5 pg  
1 hour  
5 days  
yes  
no  
no  
no  
Single copy Southern and  
Northerns  
1 hour  
1–2 hours  
High target applications  
eg. colony/plaques  
RPN2510EUM Appendix 4 Rev C 06/2007  
17  
 
 
Appendix 5. Products for  
autoradiography and  
chemiluminscent detection  
Hyperfilm – high performance autoradiography films  
Product description  
size  
number sheets  
code  
Hyperfilm ECL  
Hyperfilm ECL  
Hyperfilm ECL  
Hyperfilm ECL  
Hyperfilm ECL  
Hyperfilm ECL  
Hyperfilm ECL  
Hyperfilm MP  
Hyperfilm MP  
Hyperfilm MP  
Hyperfilm MP  
Hyperfilm MP  
Hyperfilm MP  
Hyperfilm MP  
Hyperfilm MP Enveloped  
5
18  
18  
×
7 inches  
50  
50  
100  
50  
100  
50  
50  
50  
50  
100  
50  
100  
50  
28-9068-35  
28-9068-36  
28-9068-37  
28-9068-38  
28-9068-39  
28-9068-40  
28-9068-41  
28-9068-42  
28-9068-43  
28-9068-44  
28-9068-45  
28-9068-46  
28-9068-47  
28-9068-48  
28-9068-50  
×
×
24 cm  
24 cm  
8
8
×
×
10 inches  
10 inches  
24  
35  
×
×
30 cm  
43 cm  
5
×
18  
18  
7 inches  
×
×
24 cm  
24 cm  
8
8
×
×
10 inches  
10 inches  
24  
35  
18  
×
×
×
30 cm  
43 cm  
24 cm  
50  
50  
Hypercassette™ – cassettes for autoradiography and light detection  
Size  
Code (standard)  
Code (deep)  
18  
24  
30  
35  
18  
20  
×
×
×
×
×
×
24 cm  
30 cm  
40 cm  
43 cm  
43 cm  
40 cm  
RPN11642  
RPN11643  
RPN11644  
RPN11645  
RPN11646  
RPN11647  
RPN11648  
RPN11649  
RPN11650  
RPN11628  
RPN11627  
5
8
×
×
7 inches  
10 inches  
×
RPN11629  
10 12 inches  
32  
125  
Hyperscreen™ – intensifying screens for P and  
I autoradiography  
Size  
Code  
Quantity  
18  
24  
30  
35  
18  
20  
×
×
×
×
×
×
24 cm  
30 cm  
40 cm  
43 cm  
43 cm  
40 cm  
RPN1662  
RPN1663  
RPN1664  
RPN1665  
RPN1666  
RPN1667  
RPN1668  
RPN1669  
RPN1670  
1 pair  
1 pair  
1 pair  
1 pair  
1 pair  
1 pair  
1 pair  
1 pair  
1 pair  
5
8
×
×
7 inches  
10 inches  
×
10 12 inches  
Related Products  
Product name  
Description  
Code  
Hypertorch™  
Sensitize™  
Battery powered LED darkroom torch, pack of 3  
Optimized preflash system  
RPN1620  
RPN2051  
RPN2510EUM Appendix 5 Rev C 06/2007  
18  
 
 
Appendix 6. Hybridization buffer  
Product name  
Pack size  
Code  
Rapid-Hyb buffer  
Rate enhanced hybridization buffer  
for use with radiolabelled nucleic acid probes  
125 ml  
500 m  
RPN1635  
RPN1636  
RPN2510EUM Appendix 6 Rev C 06/2007  
19  
 
 
Appendix 7: Radiation safety  
products  
Product Description  
CDC storage box  
Pipette guards  
Product Dimensions  
240 140 130 mm  
Quantity  
Code Number  
×
×
1 box  
RPN2032  
Beta pipette guard, Gilson P20/100  
Beta pipette guard, Gilson P200  
Beta pipette guard, Gilson P1000  
Multipack, 1 each of the above codes  
1 guard  
1 guard  
1 guard  
3 guards  
RPN1544  
RPN1545  
RPN1546  
RPN1556  
Beta Radiation safety starter packs  
Radioactive spills kit  
1 pack  
RPN2052  
Radioactive spills kit  
1 kit  
RPN2030  
Radioactive spills kit (US only)  
Refill pack for radioactive spills kit  
Refill pack for radioactive spills kit (US only)  
1 kit  
1 pack  
1 pack  
RPN2030US  
RPN2031  
RPN2031US  
Safety screens  
Beta safety screen, 15°, 530  
Beta safety screen, 45°, 600  
×
×
355 mm  
355 mm  
1 screen  
1 screen  
1 screen  
RPN1536  
RPN1537  
RPN2034  
Beta side/rear screen, 550  
×
350 mm  
Safety trays and liners  
Safety Trays  
685  
685  
530  
1120  
565  
455  
×
×
×
455 mm  
530 mm  
330 mm  
1 tray  
1 tray  
1 tray  
1 tray  
1 tray  
1 tray  
RPN1534  
RPN2042  
RPN2043  
RPN2063  
RPN2083  
RPN2093  
×
535 mm  
535 mm  
255 mm  
×
×
Safety Tray Liners  
to match tray 685  
to match tray 685  
to match tray 530  
×
×
×
455 mm  
530 mm  
330 mm  
25 liners  
25 liners  
25 liners  
25 liners  
25 liners  
25 liners  
RPN1528  
RPN2048  
RPN2058  
RPN2068  
RPN2088  
RPN2098  
to match tray 1120 535 mm  
×
to match tray 565  
to match tray 455  
×
×
535 mm  
255 mm  
Beta heavy-duty tube rack  
Microcentrifuge tube racks  
96  
×
35  
90  
90  
×
50 mm  
1 rack  
RPN1543  
160  
160  
×
×
×
×
75 mm (for 1.5 ml tubes)  
75 mm (for 0.5 ml tubes)  
1 rack  
1 rack  
RPN1542  
RPN2037  
Beta Waste Safes and Bags  
Beta Waste Safes  
400  
315  
150  
150  
×
×
×
×
240  
229  
120  
150  
×
×
×
×
220 mm  
230 mm  
100 mm  
150 mm  
1 safe  
1 safe  
1 safe  
1 safe  
RPN1532  
RPN2038  
RPN2039  
RPN2082  
Waste Safe Bags  
to fit RPN1532  
to fit RPN2038  
to fit RPN2082  
25 bags  
25 bags  
25 bags  
RPN2091  
RPN2090  
RPN2089  
Beta Work Tank  
540  
160  
×
×
400  
90  
×
500 mm  
1 tank  
RPN2033  
RPN1585  
Redivial workstation  
×
75 mm  
1 workstation  
Work Boxes and Safe Storage Boxes  
Work Boxes  
Beta Work Box, 185  
Beta Safe Storage Box, 300  
×
115  
×
80 mm  
185 155 mm  
1 box  
1 box  
RPN1539  
RPN1541  
×
×
Work Box Inserts  
32  
32  
32  
×
×
×
1.5 ml tubes  
2.0 ml tubes  
0.5 ml tubes  
1 insert  
1 insert  
1 insert  
RPN1540  
RPN2035  
RPN2036  
RPN2510EUM Appendix 7 Rev C 06/2007  
20  
 
 
21  
 
GE Healthcare  
GE Healthcare offices:  
GE Healthcare Bio-Sciences AB  
Björkgatan 30, 751 84 Uppsala,  
Sweden  
GE Healthcare Europe GmbH  
Munzinger Strasse 5, D-79111 Freiburg,  
Germany  
GE Healthcare Bio-Sciences Corp.  
800 Centennial Avenue, P.O. Box 1327,  
Piscataway, NJ 08855-1327,  
USA  
GE Healthcare Bio-Sciences KK  
Sanken Bldg. 3-25-1, Hyakunincho,  
Shinjuku-ku, Tokyo 169-0073,  
Japan  
For contact information for your local office,  
GE Healthcare Limited  
Amersham Place  
Little Chalfont, Buckinghamshire,  
HP7 9NA, UK  
imagination at work  
RPN2510EUM Rev C 06/2007  
 

EdgeStar Freezer FP430 FP860 User Manual
Electro Voice Speaker EVID 121 User Manual
Extron electronic Universal Remote MKP 2000 User Manual
FieldServer Computer Drive FS 8700 88 User Manual
Fluke Thermometer 5626 5628 User Manual
Frigidaire Air Conditioner P N 66121617 User Manual
Fujitsu Printer DL3800 User Manual
GE Coffeemaker 169182 User Manual
GE Coffeemaker 681131691642 User Manual
GE Water Dispenser GXSF27E User Manual